Which of the following effects is least likely to be mediated by a scaffolding protein ? increase the local concentration of signaling components. decrease allosteric effects and cooperative binding of second messengers to their targets. regulate the order in which a series of kinases can become activated following receipt of a signal. decrease likelihood of crosstalk between signaling pathways that share a common kinase. allow rapid negative feedback of a signaling response.

The correct answer is: Decrease allosteric effects and cooperative binding of second messengers to their targets.

Scaffold proteins are considered regulatory proteins that affect many signal pathways. Some of their functions are:

•Regulation and coordination of positive and negative feedback signals

•Localizing signal components within the cell (in different cell compartments)

•Tethering signaling components (assembling signal components into complexes in order to increase signal efficiency).

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