Can someone me with this question?

For #1 im not sure but the amino acids are held together with peptide bonds. For #2 The primary structure of a protein is when the amino acids are in a chain bonded with peptide bonds. This is just a string of amino acids which will then form into a sheet and combine to form a full protein

many other transport proteins, often called pumps, move materials against a concentration gradient. active transport drives molecules across a membrane from a region of lower concentration to a region of higher concentration. this process, shown in figure 5.1, uses transport proteins powered by chemi- cal energy.