How are cells affected by an isotonic hypertonic and a hypertonic solution (this is my bellwork lol)

According to the cladogram, which organisms have roundworms as a common ancestor?

Taq polymerase is an enzyme isolated from the organism thermophilus aquaticus. this organism has been found living in the hot springs of yellowstone national park. this enzyme is used to copy human dna from crime scenes. most reactions are performed at ranges similar to those of the human body; however, what considerations should be made for optimum use of this enzyme? view available hint(s)taq polymerase is an enzyme isolated from the organism thermophilus aquaticus. this organism has been found living in the hot springs of yellowstone national park. this enzyme is used to copy human dna from crime scenes. most reactions are performed at ranges similar to those of the human body; however, what considerations should be made for optimum use of this enzyme? the enzyme will not work on human dna. nothing should be altered. the ph should be decreased. the temperature should be raised.

Which direction does the energy flow in this energy pyramid ?