Regarding studies with infectious agents in animals, as the bsl and absl number increases from 1 to 4, what happens to the containment and safeguard practices?

Work in BSL-4 containment laboratory requires time and great attention. The same work that is done in a BSL-2 laboratory will take significantly longer in a BSL-4 setting. This increased time requirement is due to a lot of factors that are aimed at protecting the worker from laboratory-acquired infections. The BSL 4 laboratory should contain a Class III biological safety cabinet but may use a Class I or II BSC in combination with a positive-pressure, air-supplied full-body suit. Usually, BSL 4 laboratories are in separate buildings. Exhaust streams are filtered through high-efficiency particulate air (HEPA) filters, depending on the agents used.

there are mainly three domains in biological classification namely; archaea, bacteria, and eukarya.

archaea includes all the prokaryotic cells which are characterized by their lipid membranes. they have branched hydrocarbon which is attached to glycerol through an ether linkage.

bacteria includes all the prokaryotic cells, the cell membrane of which are made up of the unbranched fatty acid chain. in addition, they are attached to glycerol through an ester bond.

eukarya includes all the eukaryotic cells, that is, the cells contain a nucleus and other membrane-bound cell organelles.

they can be autotrophic as well as heterotrophic, unicellular as well as multicellular

they include kingdoms like protista, plantae, animalia, fungi et cetera.

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