Given are five km values for the binding of substrates to a particular enzyme. which has the strongest affinity when k–1 is greater than k2?

Answer;

- 1.2 nM

Explanation;

-Smallest Km means highest affinity, can bind substrate better at lower concentrations. The relationship between rate of reaction and concentration of substrate depends on the affinity of the enzyme for its substrate. This is usually expressed as the Km (Michaelis constant) of the enzyme, an inverse measure of affinity

-Km is therefore, the concentration of substrate which permits the enzyme to achieve half Vmax. An enzyme with a high Km has a low affinity for its substrate, and requires a greater concentration of substrate to achieve Vmax.

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