Why is it important to destroy the brain and spinal cord of a frog before conducting?

The spinal cord and the brain together comprise of the central nervous system. The spinal cord functions as a relay center for the reflex activities. The first thing the frog feels at the time of dissection is the sense of pain, and one cannot record the exact physiological reaction of the body towards the test compound.

As when one impart any stimulus, the central nervous system reacts instantaneously and starts safeguarding and reflex actions by discharging some of the chemical components and eliciting the movements of the body. The result of what one obtains in such case would not be entirely because of the test compound.

Thus, in order to inhibit the influence of the central nervous system on the contraction of the muscle, the brain and spinal cord of the frog should be destructed.

a fossil fuel is a fuel formed by natural processes, such as anaerobic decomposition of buried dead organisms, containing energy originating in ancient photosynthesis.[1] the age of the organisms and their resulting fossil fuels is typically millions of years, and sometimes exceeds 650 million years.[2] fossil fuels contain high percentages of carbon and include petroleum, coal, and natural gas.[3] other commonly used derivatives include kerosene and propane. fossil fuels range from volatile materials with low carbon to hydrogen ratios like methane, to liquids like petroleum, to nonvolatile materials composed of almost pure carbon, like anthracite coal. methane can be found in hydrocarbon fields either alone, associated with oil, or in the form of methane clathrates.

the theory that fossil fuels formed from the fossilized remains of dead plants by exposure to heat and pressure in the earth's crust over millions of years was first introduced by andreas libavius "in his 1597 alchemia [alchymia]" and later by mikhail lomonosov "as early as 1757 and certainly by 1763".[4] the first use of the term "fossil fuel" was by the german chemist caspar neumann, in english translation in 1759.[5]

in 2017 the world's primary energy sources consisted of petroleum (34%), coal (28%), natural gas (23%), amounting to an 85% share for fossil fuels in primary energy consumption in the world.[6] non-fossil sources in 2006 included nuclear (8.5%), hydroelectric (6.3%), and others (geothermal, solar, tidal, wind, wood, waste) amounting to 0.9%.[7] world energy consumption was growing at about 2.3% per year. in 2015 about 18% of worldwide consumption was from renewable sources.[8]