Which enzyme seals nicks and joins okazaki fragments? dna polymerase i dna gyrase dna primase dna ligase dna helicase?

According to theories of how life began, how did early organic molecules begin to separate from the outside world? a: specialized enzymes were required b: chains of amino acids created a barrier c: formation of microspheres or vesicles d: rna catalyzed the formation of membranes